Learn Chromatography Online: Free HPLC Training Course with AI Tutor & Certificate
You'll separate and analyze complex chemical mixtures after completing this free chromatography course. Across 11 modules and 57 video lessons, you'll master techniques like Thin Layer Chromatography (TLC), Gas Chromatography (GC), and Liquid Chromatography. In just about 1 hour, you'll build practical lab analysis skills.
Chromatography course syllabus
01 Introduction to Chromatography
- Definition and History
- Basic Principles
- Types of Chromatography
- Applications in Science and Industry
02 Paper Chromatography
- Fundamentals of Paper Chromatography
- Materials and Equipment
- Procedure and Techniques
- Analyzing Results
- Common Uses
03 Thin Layer Chromatography (TLC)
- Basics of TLC
- Preparing the TLC Plate
- Developing the Chromatogram
- Visualization Techniques
- Advantages and Limitations
04 Gas Chromatography (GC)
- Introduction to Gas Chromatography
- Components of a GC System
- Sample Preparation and Injection
- Detection Methods
- Data Interpretation
- Importance of Temperature Control in Gas Chromatography
05 Liquid Chromatography
- Overview of Liquid Chromatography
- High-Performance Liquid Chromatography (HPLC)
- Column Selection and Mobile Phase
- Detection and Analysis
- Applications in Pharmaceuticals
- Advantage of Gradient Elution in Liquid Chromatography
06 Ion Exchange Chromatography
- Principles of Ion Exchange
- Types of Ion Exchange Resins
- Process and Techniques
- Applications in Biochemistry
- Troubleshooting Common Issues
- Selectivity Determinant in Ion-Exchange Chromatography
07 Affinity Chromatography
- Concept of Affinity Chromatography
- Ligands and Binding Interactions
- Column Preparation and Loading
- Elution Strategies
- Applications in Protein Purification
- Common Application of Affinity Chromatography
08 Size Exclusion Chromatography
- Basics of Size Exclusion Chromatography
- Gel Filtration Techniques
- Calibration and Standards
- Analyzing Molecular Weight
- Applications in Polymer Science
- Principle of Size-Exclusion Chromatography
09 Advanced Chromatographic Techniques
- Multidimensional Chromatography
- Supercritical Fluid Chromatography
- Capillary Electrophoresis
- Hyphenated Techniques
- Future Trends in Chromatography
10 Safety and Best Practices
- Laboratory Safety Protocols
- Handling Chemicals and Equipment
- Waste Disposal and Environmental Considerations
- Quality Control and Assurance
- Ethical Considerations in Chromatography
11 Factors Affecting Chromatographic Resolution
- Mobile Phase Composition
- Flow Rate
- Column Characteristics
Skills you'll gain
Add these to your LinkedIn profile and resume the day you finish.
Who should take this Chromatography course?
And exactly what you need before starting (spoiler: almost nothing).
This course is for
- Chemistry and biology students looking to understand fundamental separation techniques.
- Lab technicians aiming to upgrade their skills with formal gas chromatography training.
- Quality control professionals who need a basic HPLC certificate course for their resume.
- Career switchers entering the pharmaceutical or chemical manufacturing industries in India.
Prerequisites
- No prior experience is required to begin this course.
- A basic understanding of high school chemistry concepts is helpful but not mandatory.
- An interest in laboratory techniques and chemical analysis.
How learning works on LearnTube
The structure of a bootcamp, the price of free.
AI curates your path
Our AI picks the top 1% of video content for each topic and arranges it into a structured, goal-based curriculum. No more tutorial roulette.
Learn, then get quizzed
After every lesson, your AI tutor asks you a question and gives personalised feedback on your answer, so gaps get fixed immediately.
Finish with proof
Complete the course, build the capstone project, and claim a certificate recognised by 900+ hiring partners.
A complete Chromatography course: free, structured and job-focused
You'll confidently identify chemical compounds and analyze complex mixtures by the time you finish this chromatography training. Starting with the Definition and History of the field, you'll quickly move into practical applications. The curriculum covers 11 modules that break down essential lab methods, including Paper Chromatography, Thin Layer Chromatography (TLC), and Gas Chromatography (GC). We also explore Liquid Chromatography, Ion Exchange Chromatography, and Affinity Chromatography. If you need an HPLC course online or gas chromatography training, these 57 video lessons give you a solid foundation in chemical separation.
LearnTube makes this technical subject easy to grasp. We pair curated YouTube videos with an interactive AI tutor that tests your knowledge. After every video lesson, the AI generates a quick quiz to ensure you understand concepts like Size Exclusion Chromatography, Advanced Chromatographic Techniques, and Factors Affecting Chromatographic Resolution. You can even find resources for chromatography in Hindi if you prefer learning in your native language. Best of all, the learning platform is completely free to use.
Safety and Best Practices are built directly into the curriculum so you won't mishandle lab equipment. Once you finish the 1-hour program, you can opt for a paid certificate to prove your new skills to employers. This chromatography certification is a great stepping stone if you plan to pursue specialized LC MS training or a GC MS training course in the future.
Jobs you can target after learning Chromatography
Chromatography is a gateway skill for some of the fastest-growing roles in tech.
Tests raw materials and finished products using chromatography techniques.
Prepares samples and maintains analytical equipment like GC and HPLC machines.
Supports scientists in developing new chemical compounds and running separation tests.
Typical entry-level salary ranges in India as of September 2026. Actual compensation varies by city, company and experience.
Earn a Chromatography certificate employers recognise
Finish the course and claim a certificate you can share anywhere.
- Learn 100% free: every lesson, quiz and the AI tutor cost nothing
- Claim your certificate when you complete the course (an optional paid add-on)
- Recognised by 900+ hiring partners across India
- One click to share on LinkedIn and add to your resume
Why learners choose LearnTube
Real reviews from Google. You learn free, you get assessed free, and you pay only if you want the certificate.
Though I have 11 years of experience as an HRBP, the course helped me refresh my knowledge and gave me insights into new trends in the market. The modules were clear and easy to understand, and the quizzes were well designed. I have added the certificate to my LinkedIn profile.
I had a lot of doubts while learning, but the AI chatbot answered them in detail with simple summaries. The videos on the platform saved me a lot of time as I didn't have to search YouTube and watch random content.
Just earned my certificate in HR Management from LearnTube.ai! The platform is user-friendly and the assessment was a great way to validate my knowledge. Feeling proud of this accomplishment!
I enrolled in a LearnTube personalized course to get into a Data Analytics role. It helped me learn SQL in depth and gave me hands-on experience with Power BI. I attached the LearnTube certificate to my CV, it added credibility to my profile, and I'm currently in the interview process.
The interface is user-friendly and intuitive, making it easy to navigate the course. I encountered no errors during my time using the platform, which was a refreshing change from other online learning platforms.
Just cleared the Business Analyst assessment and got certified! Loved how the course simplifies big concepts like data-driven decision making into short, practical lessons. The automated quizzes kept me on track the whole time. Perfect boost for my resume!
The certificate gave me a real edge during my B.Tech. By the time I graduated, I had skills that set me apart from my peers, and the certification opened up more job opportunities. It gave me the confidence to stand out during interviews.
I manage many software projects as a General Manager and was looking for a good certification program to stay competitive. After researching many programs, I chose LearnTube and it was much better than I expected. The material is well-organized and practical, and what I liked most is the lifetime access.
Frequently asked questions
Everything learners ask before starting this Chromatography course.
Do I need prior lab experience to take this chromatography course?
No prior lab experience is necessary. This beginner-friendly program starts with the basic definition and history of the subject. You'll gradually advance to complex methods like gas and liquid chromatography at your own pace.
What job roles can I get after completing this GC and HPLC training?
This training prepares you for entry-level roles in the pharmaceutical and chemical sectors. You can apply for positions like Quality Control Analyst, Laboratory Technician, or Research Assistant. These roles typically offer starting salaries between ₹2.0 to ₹5.0 LPA in India.
Can I study this chromatography material on my smartphone?
Yes, the LearnTube platform is fully mobile-friendly. You can watch the 57 video lessons and complete the AI tutor quizzes directly on your phone. This setup lets you learn chemical analysis techniques from anywhere.
How long does it take to finish the chromatography modules?
The entire curriculum takes about 1 hour to complete. Since the videos are self-paced, you can easily spread the 11 modules across a few days or finish the whole program in a single afternoon.
Does this program include practical HPLC method development training?
This specific course covers the theoretical foundation of liquid chromatography and advanced techniques. While it explains how the machines work, you will need physical lab access for hands-on HPLC method development and practical training.
How much are the HPLC course fees on LearnTube?
Learning on our platform is completely free. You can watch all the video lessons and take the quizzes without paying anything. You only pay a small fee if you decide to claim the optional completion certificate.
Is this gas chromatography certification course self-paced?
Yes, the entire program is self-paced. It features pre-recorded videos curated from YouTube alongside automated quizzes. You will not have to attend scheduled sessions, allowing you to study gas chromatography techniques whenever it fits your personal schedule.
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57 lessons, an AI tutor that never gets tired, and a certificate at the finish line. All you need is an hour a day.
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