Learn Hplc Online: Free Course with AI Tutor & Certificate
Designed for science students and lab technicians, this free course teaches you High-Performance Liquid Chromatography. You'll explore HPLC Instrumentation, HPLC Columns, and HPLC Method Development across 10 modules. By the end, you'll be able to operate systems, troubleshoot issues, and perform quantitative analysis confidently.
What you'll learn in this Hplc course
Five concrete outcomes you'll walk away with, each mapped to modules in the curriculum below.
Understand and apply the fundamental principles and applications of HPLC in various fields.
Operate and troubleshoot HPLC instrumentation and systems effectively.
Develop and optimize HPLC methods for accurate and reliable analysis.
Perform quantitative analysis and interpret data using advanced HPLC techniques.
Ensure safety and compliance in HPLC laboratory environments.
Hplc course syllabus
01 Introduction to HPLC
- What is HPLC?
- History and Development of HPLC
- Basic Principles of Chromatography
- Applications of HPLC
02 HPLC Instrumentation
- Components of HPLC System
- Types of HPLC Systems
- Detectors Used in HPLC
- Data Acquisition and Analysis
03 HPLC Columns
- Types of HPLC Columns
- Column Selection Criteria
- Column Maintenance and Care
- Column Troubleshooting
04 HPLC Mobile Phases
- Types of Mobile Phases
- Preparation of Mobile Phases
- Mobile Phase Selection
- Mobile Phase Troubleshooting
05 Sample Preparation for HPLC
- Importance of Sample Preparation
- Techniques for Sample Preparation
- Sample Filtration and Degassing
- Sample Injection Methods
06 HPLC Method Development
- Steps in Method Development
- Optimization of HPLC Methods
- Validation of HPLC Methods
- Documentation and Reporting
07 HPLC Troubleshooting
- Common HPLC Problems
- Troubleshooting Techniques
- Preventive Maintenance
- Case Studies in Troubleshooting
08 Quantitative Analysis using HPLC
- Calibration and Standardization
- Quantitative Techniques
- Data Interpretation
- Quality Control in HPLC
09 Advanced HPLC Techniques
- Gradient Elution Techniques
- Chiral HPLC
- HPLC-MS (Mass Spectrometry)
- High-Throughput HPLC
10 Safety and Compliance in HPLC
- Safety Practices in HPLC Labs
- Regulatory Compliance
- Handling Hazardous Materials
- Waste Disposal and Environmental Considerations
Real-world projects you'll work on
Practice the exact kind of work Hplc professionals do on the job.
Developing a High-Throughput HPLC Method for Pharmaceutical Quality Control at Pfizer
Optimizing Chiral HPLC Techniques for Enantiomer Separation in Novartis Drug Development
Implementing Advanced HPLC-MS for Environmental Pollutant Analysis at BASF
Designing a Comprehensive HPLC Troubleshooting Guide for Roche Laboratories
Skills you'll gain
Add these to your LinkedIn profile and resume the day you finish.
Who should take this Hplc course?
And exactly what you need before starting (spoiler: almost nothing).
This course is for
- Chemistry and pharmacy students wanting practical lab skills.
- Working lab technicians aiming to upgrade their analytical techniques.
- Quality control analysts preparing for roles in the Indian pharmaceutical industry.
- Career switchers moving into environmental analysis or drug development.
Prerequisites
- No prior experience with HPLC is required to begin this course.
- A basic understanding of chemistry or laboratory science is helpful.
- Access to a computer and internet connection for viewing lessons.
How learning works on LearnTube
The structure of a bootcamp, the price of free.
AI curates your path
Our AI picks the top 1% of video content for each topic and arranges it into a structured, goal-based curriculum. No more tutorial roulette.
Learn, then get quizzed
After every lesson, your AI tutor asks you a question and gives personalised feedback on your answer, so gaps get fixed immediately.
Finish with proof
Complete the course, build the capstone project, and claim a certificate recognised by 900+ hiring partners.
A complete Hplc course: free, structured and job-focused
Ideal for chemistry students, aspiring analytical chemists, and lab professionals, this free course teaches you the fundamental principles and advanced applications of High-Performance Liquid Chromatography. You'll start with a solid Introduction to HPLC and move through essential topics like HPLC Instrumentation, HPLC Columns, and HPLC Mobile Phases. As you progress, the curriculum covers Sample Preparation for HPLC, HPLC Method Development, and HPLC Troubleshooting. You'll also tackle Quantitative Analysis using HPLC and Advanced HPLC Techniques, finishing up with Safety and Compliance in HPLC.
To build real-world experience, you'll work on practical projects based on actual industry scenarios. These include developing a high-throughput HPLC method for pharmaceutical quality control at Pfizer, optimizing chiral techniques for enantiomer separation at Novartis, implementing HPLC-MS for environmental pollutant analysis at BASF, and designing a troubleshooting guide for Roche Laboratories. These hands-on tasks ensure you understand how to apply your skills directly in professional labs.
LearnTube makes mastering these skills simple and highly interactive. We curate the best YouTube video lessons and pair them with an AI tutor that gives you a quick quiz after every single lesson to check your understanding. Learning is completely free, and you can choose to add a paid certificate at the end to verify your new skills for employers.
Jobs you can target after learning Hplc
Hplc is a gateway skill for some of the fastest-growing roles in tech.
Operates HPLC equipment to analyze chemical compounds and ensure product quality.
Tests raw materials and finished products in pharmaceutical labs using chromatography.
Develops and validates new analytical methods for drug discovery and development.
Maintains lab instruments, prepares samples, and assists with routine HPLC testing.
Typical entry-level salary ranges in India as of November 2026. Actual compensation varies by city, company and experience.
Earn a Hplc certificate employers recognise
Finish the course and claim a certificate you can share anywhere.
- Learn 100% free: every lesson, quiz and the AI tutor cost nothing
- Claim your certificate when you complete the course (an optional paid add-on)
- Recognised by 900+ hiring partners across India
- One click to share on LinkedIn and add to your resume
Why learners choose LearnTube
Real reviews from Google. You learn free, you get assessed free, and you pay only if you want the certificate.
Though I have 11 years of experience as an HRBP, the course helped me refresh my knowledge and gave me insights into new trends in the market. The modules were clear and easy to understand, and the quizzes were well designed. I have added the certificate to my LinkedIn profile.
I had a lot of doubts while learning, but the AI chatbot answered them in detail with simple summaries. The videos on the platform saved me a lot of time as I didn't have to search YouTube and watch random content.
Just earned my certificate in HR Management from LearnTube.ai! The platform is user-friendly and the assessment was a great way to validate my knowledge. Feeling proud of this accomplishment!
I enrolled in a LearnTube personalized course to get into a Data Analytics role. It helped me learn SQL in depth and gave me hands-on experience with Power BI. I attached the LearnTube certificate to my CV, it added credibility to my profile, and I'm currently in the interview process.
The interface is user-friendly and intuitive, making it easy to navigate the course. I encountered no errors during my time using the platform, which was a refreshing change from other online learning platforms.
Just cleared the Business Analyst assessment and got certified! Loved how the course simplifies big concepts like data-driven decision making into short, practical lessons. The automated quizzes kept me on track the whole time. Perfect boost for my resume!
The certificate gave me a real edge during my B.Tech. By the time I graduated, I had skills that set me apart from my peers, and the certification opened up more job opportunities. It gave me the confidence to stand out during interviews.
I manage many software projects as a General Manager and was looking for a good certification program to stay competitive. After researching many programs, I chose LearnTube and it was much better than I expected. The material is well-organized and practical, and what I liked most is the lifetime access.
Frequently asked questions
Everything learners ask before starting this Hplc course.
How is this different from watching regular YouTube videos?
Unlike plain video platforms, LearnTube organizes curated videos into a structured 10-module syllabus. You'll get an AI tutor that tests your knowledge with interactive quizzes after every lesson, ensuring you actually retain the material.
What software or tools do I need installed for this course?
You do not need to install any specialized chromatography software. A standard web browser on your phone or computer is all you need to watch the lessons, take the quizzes, and complete the coursework.
Do I need prior lab experience to take this course?
No prior hands-on experience with chromatography is required. The course starts with an Introduction to HPLC, making it accessible for beginners, though a basic understanding of general chemistry principles will help you grasp the concepts faster.
What kind of jobs and salaries can this training lead to?
This training prepares you for roles like Quality Control Analyst and Analytical Chemist. In India, entry-level professionals in these positions typically earn between ₹2.5 LPA and ₹5 LPA, especially within the pharmaceutical and environmental testing sectors.
Is the learning material self-paced or scheduled?
The entire course is completely self-paced. You can log in and study the modules whenever you have free time, allowing you to balance your education with work, college, or other personal commitments.
Will I get a certificate after completing the modules?
Yes, a certificate is available. While all the video lessons and AI quizzes are completely free, you can choose to purchase an optional paid certificate at the end to prove your new skills to employers.
Are there practical projects included in the curriculum?
Yes, the course includes several hands-on projects. You'll work on industry-inspired scenarios, such as developing a high-throughput method for Pfizer and creating a troubleshooting guide for Roche Laboratories, to build practical confidence.
Learners also explore these free courses
Pair Hplc with a complementary skill to stand out even more.
Start learning Hplc today, for free
40 lessons, an AI tutor that never gets tired, and a certificate at the finish line. All you need is an hour a day.
Start Learning Free